In particular, bsAbs are often associated with a higher level of impurities and byproducts, including aggregates, fragments and mispaired products (Garber 2014; Taki et al. 2015; Andrade et al. 2019; Michaelson et al. 2009; Michaelson et al. 2009; Jakobsen et al. 2011, Klein et al. 2012), with the generally lower titers of bsAbs often translating to relatively higher host cell protein (HCP) levels. We (Chen et al. 2022), along with others (Tustian et al. 2016, 2018; Lindhofer et al. 1995; Smith et al. 2015; Zwolak et al. 2017a, b; Zwolak et al. 2017a, b; Skegro, et al. 2017; Ollier et al. 2019; Chen et al. 2020; Zhang et al. 2021), have reported the effective use of Protein A as a capture step to effectively remove such impurities and byproducts. An effective polishing strategy is, however, irreplaceable, as > 1% of high molecular weight (HMW) species and > 100 ppm HCP often remains after the first Protein A capture step. In addition, further impurities such as leached protein A, potential increase in aggregates after viral inactivation steps or supplementary viral removal necessitate the presence of further polishing steps. If your hardware has a highly glossed finish, your attempts to clean the subsurface material will fail. To remove clear coats and get to the nitty gritty, you’ll want to use mineral spirits or another solvent. You can always reapply the clear coat after the cleaning process. Two Easy At-Home Cleaning Solutions for Brass and Copper Door Knobs Recipe #1: Steel wool is the most painless way to strip spray paint off metal hardware at home. Choose fine grit #0000 steel wool from your neighborhood hardware store. Oldenburg J, Mahlangu JN, Kim B, Schmitt C, Callaghan MU, Young G et al (2017) Emicizumab prophylaxis in hemophilia A with inhibitors. N Engl J Med 377(9):809–818 Igawa T, Tsunoda H. (2009). Methods of modifying antibodies for purification of bispecific antibodies. US20090263392 A1, 22 Oct 2009.

Dish soap is a mild soap that cuts through dirt and grease, so it’s ideal for cleaning bronze or stainless steel door pieces. Mix a tablespoon of liquid dish soap into three cups of warm water to make a DIY brass cleaning and polishing solution for door knobs and hardware in your home. Most general-purpose cleaning solutions will adequately clean crystal, glass and clear plastic knobs and handles. However, use caution to avoid those that contain solvents that can damage your hardware. Its solutions for the most general-purpose cleaning will clean enough for clean plastics, crystals, glass, knobs, and handles. But, you have to be careful always. Because there are solvents on the market that can damage your hardware, be careful not to overdo it.Modern brass is usually lacquered with a clear finish to stop it discolouring and by comparison to old brass looks yellowish in colour. Cleaning modern brass is easy, simply wipe with a soft damp cloth. If it’s really grimy, use a mild mix of washing up liquid and warm water. Wipe the surface clean, wipe with a clean cloth and dry thoroughly. Brass cleaning home-remedies And remember that if you wash with a brush, any sharp spots will be easily removed. Once the rag is clean, rinse with soap and water to clean it. Make sure you remove all soap residue in it. Then clean and dry with a piece of pure fabric. 5. Clean Using Vinegar

Cast and malleable iron products are always at risk of corroding and rusting, more so if used externally. Amersham HCPQuant CHO kit (Cytiva) was used according to manufacturer’s instructions to determine the CHO HCP content, with data acquisition performed on the Synergy™ 2 plate reader (BioTek). If you follow the rest of this tutorial, you should rinse all the WD-40 off your knobs and pulls before reinstalling, but I still feel that I need to post this warning…. Step 3: Soak Kitchen Cabinet Knobs and Pulls in Vinegar Solution

Moreover, regular polishing of the surface of the handles is also recommended as it protects the metal from corrosion. Apply a coat of a wax-based product like carnauba wax on the surface of your brass handles once in three months to ensure they are adequately lubricated, and to inhibit unwanted tarnishing. Conclusion Additionally, use a protective coating on the brass to prevent scratches and mild corrosive damage, making it easier for you to maintain their beautiful sheen. With a little effort, your brass door handles will look great for years to come! Preparing to clean You can also slice a lemon in half, sprinkle salt over the cut section, and rub it on the tarnished surface until clean. Avoid rubbing too hard, which may cause scratching, and clean the handle with water and a microfiber cloth. Gökbuget N, Dombret H, Bonifacio M, Reichle A, Graux C, Faul C et al (2018) Blinatumomab for minimal residual disease in adults with B-cell precursor acute lymphoblastic leukemia [correction (2019) Blood 133(24):2625]. Blood 131(14):1522–1531 For the selected polishing resins, Capto Butyl ImpRes is a hydrophobic resin, Capto adhere ImpRes is a multimodal anion exchanger with ionic interactions, hydrogen bonding and hydrophobic interactions, while Capto S ImpAct is a strong cation exchanger. Based on the above stability study results, only Capto S ImpAct is feasible to be explored as bind–elute mode polishing, as both Capto Butyl ImpRes and Capto adhere ImpRes require relatively high-salt conditions beyond the stable range of both bsAb molecules used in this study for bind–elute mode. Therefore, we decided to explore flowth-rough mode polishing instead for Capto Butyl ImpRes and Capto adhere ImpRes.

Bakelite is an early plastic, developed at the start of the 20th century. It was one of the first synthetic plastics ever to be produced. All those beautiful old radios that you see for sale in antique stores are most likely made from Bakelite. Also made from Bakelite was a lot of electrical appliances such as light switches, telephones, and also wire insulation. The reason why it worked so well was it's high resistance - not only to electricity, but to heat and chemical action. Sharkey B, Pudi S, Moyer IW, Zhong L, Prinz B, Baruah H et al (2017) Purification of common light chain IgG-like bispecific antibodies using highly linear pH gradients. Mabs 9(2):257–268 Sometimes, all you need is mild dish soap to get a door knob clean, while other times, it may be necessary to use a bleach solution to kill germs and bacteria. Learn how to use various cleaners for different handles to leave them shiny and clean. Cleaning Door Knobs Made of Different Materials If you do not use a combo product for polishing, follow the instructions in the manual. Use a soft fabric to lighten and polish the doorknob. You have confirmed all parts and holes in the handle during buffing. If your brass is undisturbed, finish with mineral oil for protection. Brass and copper are two traditional door handle and hinge materials that are seeing a resurgence in popularity in modern homes. While they are susceptible to tarnishing, they can easily be cleaned with a few household products and a bit of elbow grease. However, there are two essential things to ask yourself before you get started:Spiesberger K, Paulfranz F, Egger A, Reiser J, Vogl C, Rudolf-Scholik J et al (2015) Large-scale purification of r28M: a bispecific scFv antibody targeting human melanoma produced in transgenic cattle. PLoS ONE 10(10):e0140471

All external or exposed surfaces require regular / weekly cleaning with a soft cloth, using warm water and mild detergent to remove built-up dirt.

Ok - enough of the history lesson. This Instructable is about how to restore Bakelite to its original shine and lustre. Over time Bakelite starts to oxidise due to sunlight and over time will become dull. The surface has a thin layer of oxidised material which can be removed with some careful cleaning. Some brass is lacquered to protect its surface and prevent tarnishing. The best way to clean lacquered brass is to simply wipe it with a soft, damp cloth. Don’t use household cleaner on it or you will damage the surface. How to clean new brass Stainless steel is a fantastic metal for door handles, and unlike other materials, cannot wear away with excessive cleaning. The way that stainless steel protects itself from corrosion is actually the chromium added to the steel, which bonds with oxygen in the air (which would normally cause rust in iron-based metals) to form a passive barrier of chromium oxide on the surface of your stainless steel door handles. Tustian AD, Laurin L, Ihre H, Tran T, Stairs R, Bak H (2018) Development of a novel affinity chromatography resin for platform purification of bispecific antibodies with modified protein a binding avidity. Biotechnol Prog 34(3):650–658 Taylor K, Howard CB, Jones ML, Sedliarou I, MacDiarmid J, Brahmbhatt H et al (2015) Nanocell targeting using engineered bispecific antibodies. Mabs 7(1):53–65